Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9auc
Q-score: 0.503
Cao J, Belousoff MJ, Danev R, Christopoulos A, Wootten D, Sexton PM
Biochemistry (2024) [ DOI: doi:10.1021/acs.biochem.4c00114 Pubmed: 38603770 ]
- Human amylin 1 receptor in complex with gs and human calcitonin gene-related peptide (Complex from Homo sapiens)
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Calcitonin gene-related peptide 1 (3 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
SPACEtomo example tomograms of Baker's Yeast
Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ Pubmed: 37673901 DOI: doi:10.1038/s41467-023-41199-5 ]
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Xanomeline (281 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f0j
Q-score: 0.621
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcitonin receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 8f0k
Q-score: 0.564
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- Human amylin 3 receptor in complex with gs protein and san385 peptide (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- San385 (3 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8f2a
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- Pramlintide analogue san385 (3 kDa, Protein from Homo sapiens)
- Human amylin 3 receptor in complex with gs protein and san385 peptide in another conformation (cluster 5) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8f2b
Q-score: 0.556
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2024) 20 pp. 162-169 [ DOI: doi:10.1038/s41589-023-01393-4 Pubmed: 37537379 ]
- N-hexadecanoyl-l-glutamic acid (385 Da, Ligand)
- Pramlintide analogue san45 (3 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- (2s)-2-{[(1r)-1-hydroxyhexadecyl]oxy}-3-{[(1r)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate (766 Da, Ligand)
- Calcitonin receptor (58 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Receptor activity-modifying protein 3 (16 kDa, Protein from Homo sapiens)
- Amylin 3 receptor in complex with gs protein and san45 peptide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Apoferritin at 92% of super resolution sampling limit (200KV CRYOARM + K3 with gold grids)
Burton-Smith RN, Danev R, Murata K
To Be Published
- Apoferritin (500 kDa, Complex from Mus musculus)
Apoferritin at 100% of super resolution sampling limit (Krios G4 + Falcon 4 with copper grids)
Burton-Smith RN, Danev R, Murata K
To Be Published
- Apoferritin (500 kDa, Complex from Mus musculus)
Apoferritin at 113% of super resolution sampling limit (Krios G4 + Falcon 4 with copper grids)
Burton-Smith RN, Danev R, Murata K
To Be Published
- Apoferritin (500 kDa, Complex from Mus musculus)